Pseudomonas and Nonfermenters

Июль 31, 2022

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Биология
Pseudomonas and Nonfermenters

Содержание

2. Pseudomonas and Nonfermenters
3. Opportunistic Pathogens of Plants, Animals, and Humans Many Taxonomic Changes in Last Decade Clinically Important Aerobic
4. General Characteristics of Nonfermenters Oxidative gram-negative bacilli, including Pseudomonas spp., produce acid from glucose or other
5. Clinically Important Nonfermentative Gram-Negative Bacilli Later Lab only
7. Pseudomonas aeruginosa (Family Pseudomonadaceae)
8. Motile (by single or multiple polar flagella) gram-negative rods Obligate (strict) aerobes (most strains) Oxidase (usually)
9. Pseudomonas aeruginosa Infections
10. Pseudomonas aeruginosa Infections (cont.) Characteristic grape-like odor. Bluish-green color clinically and in the lab due to
11. Virulence Factors Associated with Pseudomonas aeruginosa
12. Mechanism of Action of Exotoxin A
13. Mechanisms of Antibiotic Resistance in Pseudomonas aeruginosa
15. Burkholderia cepacia
16. Diseases Associated with Burkholderia spp.
17. Stenotrophomonas maltophilia
18. Stenotrophomonas maltophilia
19. Stenotrophomonas maltophilia (cont.)
20. Stenotrophomonas maltophilia (cont.)
21. Acinetobacter baumanii
22. Acinetobacter baumanii
23. Moraxella catarrhalis
24. Moraxella catarrhalis
26. REVIEW Pseudomonas and Nonfermenters
27. General Characteristics of Nonfermenters Oxidative gram-negative bacilli, including Pseudomonas spp., produce acid from glucose or other
28. Clinically Important Nonfermentative Gram-Negative Bacilli Later Lab only REVIEW
29. Review of Pseudomonas aeruginosa (Family Pseudomonadaceae)
30. Motile (by single or multiple polar flagella) gram-negative rods Obligate (strict) aerobes (most strains) Oxidase (usually)
31. Pseudomonas aeruginosa Infections REVIEW
32. Pseudomonas aeruginosa Infections (cont.) Characteristic grape-like odor. Bluish-green color clinically and in the lab due to
33. Virulence Factors Associated with Pseudomonas aeruginosa REVIEW
34. Mechanism of Action of Exotoxin A REVIEW
35. Mechanisms of Antibiotic Resistance in Pseudomonas aeruginosa REVIEW
36. Review of Burkholderia cepacia
37. Diseases Associated with Burkholderia spp. REVIEW
38. Review of Stenotrophomonas maltophilia
39. Stenotrophomonas maltophilia (cont.) REVIEW
40. Review of Acinetobacter baumanii
41. Acinetobacter baumanii REVIEW
42. Review of Moraxella catarrhalis
43. Moraxella catarrhalis REVIEW
45. Скачать презентацию

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Pseudomonas and Nonfermenters

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Opportunistic Pathogens of Plants, Animals, and Humans
Many Taxonomic Changes in

Last Decade
Clinically Important Aerobic Gram-Negative Bacilli Include:
Aerobic nonfermenters: 10-15% of clinical isolates
Pseudomonas aeruginosa; Burkholderia cepacia; Stenotrophomonas maltophilia; Acinetobacter baumannii; Moraxella catarrhalis: Account for >75% of all clinical isolates of aerobic nonfermenters
Facultative anaerobes and microaerophiles: 70-80% of clinical isolates
Haemophilus & related organisms: 10-15% of clinical isolates
Unusual bacilli: <1% of clinical isolates
Pseudomonads Classified into Five rRNA Groups

General Overview

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General Characteristics of Nonfermenters
Oxidative gram-negative bacilli, including Pseudomonas spp., produce acid

from glucose or other carbohydrates only in the presence of oxygen (nonfermenters).
NOTE: Enterobacteriaceae, Aeromonas and Vibrio are fermentative and can utilize carbohydrates in the absence of oxygen.
Pseudomonas aeruginosa oxidizes but does not ferment glucose. Alcaligenes faecalis neither ferments nor oxidizes glucose (see Lab Manual).

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Clinically Important Nonfermentative Gram-Negative Bacilli
Later
Lab only

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Pseudomonas aeruginosa
(Family Pseudomonadaceae)

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Motile (by single or multiple polar flagella) gram-negative rods
Obligate (strict)

aerobes (most strains)
Oxidase (usually) and catalase positive
Nonfermentative chemoheterotrophic respiratory metabolism
Minimal nutritional reqts.; Many organic compounds used as C and N sources, but only a few carbohydrates by oxidative metabolism
Glucose used oxidatively
Lactose negative on MacConkey’s agar
Some strains produce diffusible pigments:
Pyocyanin (blue); fluorescein (yellow); pyorubin (red)
P. aeruginosa produces characteristic grape-like odor and blue-green pus & colonies
Broad antibiotic resistance

Characteristics of Pseudomonas aeruginosa

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Pseudomonas aeruginosa Infections

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Pseudomonas aeruginosa Infections (cont.)
Characteristic grape-like odor. Bluish-green color clinically and in

the lab due to presence of two pigments: pyocyanin & fluorescein.

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Virulence Factors Associated with Pseudomonas aeruginosa

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Mechanism of Action of Exotoxin A

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Mechanisms of Antibiotic Resistance in Pseudomonas aeruginosa

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Burkholderia cepacia

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Diseases Associated with Burkholderia spp.

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Stenotrophomonas maltophilia

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Stenotrophomonas maltophilia

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Stenotrophomonas maltophilia (cont.)

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Stenotrophomonas maltophilia (cont.)

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Acinetobacter baumanii

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Acinetobacter baumanii

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Moraxella catarrhalis

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Moraxella catarrhalis

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REVIEW
Pseudomonas and Nonfermenters

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General Characteristics of Nonfermenters
Oxidative gram-negative bacilli, including Pseudomonas spp., produce acid

from glucose or other carbohydrates only in the presence of oxygen (nonfermenters).
NOTE: Enterobacteriaceae, Aeromonas and Vibrio are fermentative and can utilize carbohydrates in the absence of oxygen.
Pseudomonas aeruginosa oxidizes but does not ferment glucose. Alcaligenes faecalis neither ferments nor oxidizes glucose (see Lab Manual).

REVIEW

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Clinically Important Nonfermentative Gram-Negative Bacilli
Later
Lab only
REVIEW

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Review of Pseudomonas aeruginosa
(Family Pseudomonadaceae)

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Motile (by single or multiple polar flagella) gram-negative rods
Obligate (strict)

aerobes (most strains)
Oxidase (usually) and catalase positive
Nonfermentative chemoheterotrophic respiratory metabolism
Minimal nutritional reqts.; Many organic compounds used as C and N sources, but only a few carbohydrates by oxidative metabolism
Glucose used oxidatively
Lactose negative on MacConkey’s agar
Some strains produce diffusible pigments:
Pyocyanin (blue); fluorescein (yellow); pyorubin (red)
P. aeruginosa produces characteristic grape-like odor and blue-green pus & colonies
Broad antibiotic resistance

Characteristics of Pseudomonas aeruginosa

REVIEW

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Pseudomonas aeruginosa Infections
REVIEW

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Pseudomonas aeruginosa Infections (cont.)
Characteristic grape-like odor. Bluish-green color clinically and in

the lab due to presence of two pigments: pyocyanin & fluorescein.

REVIEW

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Virulence Factors Associated with Pseudomonas aeruginosa
REVIEW

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Mechanism of Action of Exotoxin A
REVIEW

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Mechanisms of Antibiotic Resistance in Pseudomonas aeruginosa
REVIEW

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Review of Burkholderia cepacia

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Diseases Associated with Burkholderia spp.
REVIEW

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Review of Stenotrophomonas maltophilia

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Stenotrophomonas maltophilia (cont.)
REVIEW

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Review of Acinetobacter baumanii

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Acinetobacter baumanii
REVIEW

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Review of Moraxella catarrhalis

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Moraxella catarrhalis
REVIEW