Comparison of Competitors’ Evaporative Light Scattering Detectors

Август 9, 2022

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Comparison of Competitors’ Evaporative Light Scattering Detectors

Содержание

2. What affects ELSD performance? Nebulization Size of droplet formed: consequently determines size of particle Evaporation Efficiency
3. Nebulization Increase Neb gas flow ----------- Reduces droplet size Increase Liquid flow --------------- Increases droplet size
4. Over the last four years, the number of ELSD manufacturers has increased considerably. Current ELSD manufacturers
5. Alltech ELSD 800 Light source: 670nm @ 5mW laser diode Detector element: Silicon photodiode Temperature range:
6. Alltech ELSD 2000ES Light source: 650nm 30mW Laser diode Detector element: Silicon photodiode Temperature range: Ambient
7. Alltech ELSD 3300ES Light source: 650nm 30mW Laser diode Silicon photodiode detector Temperature range: Ambient to
8. Nebulizer Gas flow adjusted for solvent type No nebulizer temperature control Evaporation Temperature setting dependent on
9. SEDEX 85 LT-ELSD Blue light source Expanded software functionality Mass flow controller Remote power-down Automatic gas
10. Nebulizer 3.0 SLMs of gas used to nebulize solvents under standard conditions Large volume nebulizer chamber
11. Evaporation Long coiled tube which removes large droplets by centrifugal force and provides laminar flow -
12. ELSD 2040 Light Source: Tungsten Halogen Lamp Detector Element: PMT Temperature Range: Ambient to 100°C (?)
13. Acquity ELSD Designed for UPLC an analysis 80Hz Data rate Sharp peak shape (1.2 - 1.5sec)
14. Nebulizer Snap-on design for easy replacement Temperature controllable (% of drift tube temp) Gas pressure/flow set
15. ELSD 400 Light source: 670nm LASER Detector element: Photodiode Temperature range: Ambient to 120°C Gas inlet
16. Nebuliser: Thermo-split Technology For difficult to evaporate mobile phases, or high flow rates, the nebulizer chamber
17. ZAM 3000 Light source: Tungsten Halogen Detector element: Photomultiplier Temperature range: Ambient to 70°C Nebulizer gas
18. Nebulizer For difficult to evaporate mobile phases, or high flow rates, the nebulizer chamber walls are
19. Chromachem ELSD Light source: Halogen lamp Detector element: Photomultiplier Temperature range: Nebulizer: Up to 70°C by
20. Nebulizer Similar design to SEDEX 75 Evaporation Similar design to SEDEX 75 No special design features
21. ESA’s Charged Aerosol Detector (CAD)
22. How it works: The column eluent is nebulized with nitrogen, before passing into a heated drift
23. This is a non-optical technique and therefore is not bound by scattering laws. Small particles, which
24. The technique is dependent on nebulization and evaporation stages. It is gradient sensitive - the response
25. Overview of ELSD Sales Global ELSD Market - ca. 1000-1500 units/year PL shares ca. 25% of
26. ELSD Sales by Region
27. Competitor Overview
28. Competitor Overview
30. Скачать презентацию

Слайд 2

What affects ELSD performance?
Nebulization
Size of droplet formed: consequently determines size

of particle
Evaporation
Efficiency to remove solvent
Light source
Intensity
Wavelength

Слайд 3

Nebulization
Increase Neb gas flow ----------- Reduces droplet size
Increase Liquid flow ---------------

Increases droplet size
Increase Liquid viscosity -------- Increases droplet size
Ideally, you need the largest droplet size possible, but not too large that it can’t be fully evaporated in the drift tube.
Hence, the efficiency of your evaporation step is critical to the performance of an ELSD.

Слайд 4

Over the last four years, the number of ELSD manufacturers has

increased considerably.
Current ELSD manufacturers are:
Alltech
Sedere
Waters
SofTA
Schambeck
ESA
Polymer Labs

Current Manufacturers of ELS Detectors

Слайд 5

Alltech
ELSD 800
Light source: 670nm @ 5mW laser diode
Detector element: Silicon

photodiode
Temperature range: Ambient to 110°C
Nebulizer gas: Up to 3.0L/min,
Gas inlet pressure: 15–90psi (1.0–6.0 Bar)
Typical operating range: 1.0-3.0L/min
Mobile phase flow rate: 1–5.0mL/min
Analog outputs: 1V or 10mV full scale

Слайд 6

Alltech
ELSD 2000ES
Light source: 650nm 30mW Laser diode
Detector element: Silicon

photodiode
Temperature range: Ambient to 120°C
Nebulizer gas: Up to 4.0L/min (MFC controlled)
Gas inlet pressure: 60–80psi
Typical operating range: 1.0-3.0L/min
Low temperature operation
The optical and electronic components have been redesigned to minimize background noise and increase sensitivity.

Слайд 7

Alltech
ELSD 3300ES
Light source: 650nm 30mW Laser diode
Silicon photodiode detector
Temperature

range: Ambient to 120°C
Nebulizer gas: Up to 4.0L/min (MFC controlled)
Gas inlet pressure: 60–80psi
Typical operating range: 1.0-3.0L/min
Low temperature operation
The optical and electronic components have been redesigned to minimize background noise and increase sensitivity.

Слайд 8

Nebulizer
Gas flow adjusted for solvent type
No nebulizer temperature control
Evaporation
Temperature

setting dependent on solvent properties
Impactor removes the large droplets at low temperatures -limits to how low in temperature can go
Detection
Laser source @ 650-670nm - scattering efficiency is lower at higher wavelengths but this is compensated by 30mW LASER
Silicon photodiode detector - optimum sensitivity to red light

Design Features of Alltech’s ELSDs

Слайд 9

SEDEX 85 LT-ELSD
Blue light source
Expanded software functionality
Mass flow controller
Remote power-down
Automatic

gas shut-off

SEDEX 75 LT-ELSD
Tungsten halogen lamp
Coiled drift tube
No mass flow controller
Different nebulizers for different flow rates

Design Features of Sedere’s ELSDs

Слайд 10

Nebulizer
3.0 SLMs of gas used to nebulize solvents under

standard conditions
Large volume nebulizer chamber with a bend removes large droplets
Nebulizer not temperature controlled
cannot connect to SFC systems without modification
Different size nebulizers for different flow rates

Design Features of Sedere’s ELSDs

Слайд 11

Evaporation
Long coiled tube which removes large droplets by centrifugal force and

provides laminar flow - potential increase in band broadening
Longer drift tube means the temperature can be lower, compared to a short tube, for a given solvent
Longer tubes have greater equilibration times for heating and cooling
Detection
SEDEX 75 Halogen lamp produces a broad range of wavelengths, but low intensity
SEDEX 85 uses a blue LED, same as PL-ELS 2100
PMT gain adjustable

Design Features of Sedere’s ELSDs

Слайд 12

ELSD 2040
Light Source: Tungsten Halogen Lamp
Detector Element:

PMT
Temperature Range: Ambient to 100°C (?)
Nebulizer Gas: adjustable 3-60psi,
Nebulizer set at 0-100% of Drift tube temp
Gas Inlet Pressure: 65psi min
High and Low flow nebulizers
0.050-3ml/min
2 Analog Outputs: 2V full scale
Digital Output

Waters

Слайд 13

Acquity ELSD
Designed for UPLC an analysis
80Hz Data rate
Sharp peak shape

(1.2 - 1.5sec)
Stackable
Digital Output

Waters

Слайд 14

Nebulizer
Snap-on design for easy replacement
Temperature controllable (% of drift tube

temp)
Gas pressure/flow set according to solvent
Evaporation
Coiled drift tube
No design features included to operate at low temperatures
Detection
Halogen lamp – high power, but produces a wide range of wavelengths (mean wavelength ca. 900nm)
PMT – detector type not optimized for light source
Heated optics, to prevent condensation onto optical lenses (same as PL-ELS 2100)

Design Features of Waters’ ELSDs

Слайд 15

ELSD 400
Light source: 670nm LASER <5mW
Detector element: Photodiode
Temperature range:

Ambient to 120°C
Gas inlet pressure 50psi
Nebulizer chamber 0-80°C
Analog Output: 0-1V full scale
Digital Output
Heated/Cooled Nebulizer Chamber (Thermosplit)

SofTA

Слайд 16

Nebuliser: Thermo-split Technology
For difficult to evaporate mobile phases, or high

flow rates, the nebulizer chamber walls are cooled
By making the walls suitably cold, 99+% of an aqueous stream can be diverted away from the evaporative zone
Nebulizer gas pressure set according to solvent type (no MFC)
Evaporation
Heated drift tube (coiled ??) no form of impactor or diffuser
Detection
LASER diode – same as Alltech
Photodiode detector

Design Features of SofTA’s ELSDs

Слайд 17

ZAM 3000
Light source: Tungsten Halogen
Detector element: Photomultiplier

Temperature range: Ambient to 70°C
Nebulizer gas flow: <1L/min
Drying gas: <2L/min
Analog output: 0-1V full scale
Digital output

Schambeck

Слайд 18

Nebulizer
For difficult to evaporate mobile phases, or high flow

rates, the nebulizer chamber walls are cooled
By making the walls suitably cold, 99+% of an aqueous stream can be diverted away from the evaporative zone
Nebulizer gas pressure set according to solvent type (no MFC)
Evaporation
Heated glass drift tube no form of impactor or diffuser
Detection
Tungsten Halogen Lamp (av. wavelength ca. 900nm)
Photomultiplier tube - not optimized for light source

Design Features of Schambeck’s ELSDs

Слайд 19

Chromachem ELSD
Light source: Halogen lamp
Detector element: Photomultiplier
Temperature range:

Nebulizer: Up to 70°C by 1°C increments
Evaporator: Up to 150°C by 1°C increments
Gas inlet pressure: 0.5 to 3.9 bars (1 to 4 L/min) Helium or Nitrogen
Mobile phase flow rate: 50µl/min to 4 ml/min
Digital outputs: RS232

ESA

Слайд 20

Nebulizer
Similar design to SEDEX 75
Evaporation
Similar design to SEDEX

75
No special design features for low temperature operation
Detection
Tungsten-Halogen lamp (wavelength ca. 900nm)
Photomultiplier tube - not optimized for light source

Design Features of ESA’s ELSDs

Слайд 21

ESA’s Charged Aerosol Detector (CAD)

Слайд 22

How it works:
The column eluent is nebulized with nitrogen, before passing

into a heated drift tube.
The droplets are dried to remove mobile phase, producing analyte particles.
Dried particles are mixed with a secondary stream of positively charged nitrogen as it passes a high-voltage, platinum corona wire.
This charged gas subsequently imparts a charge onto the stream of analyte particles.
These streams of charged particles then pass through a charge collector where the magnitude of the imparted charge is measured by a electrometer.
A signal is generated which is proportional to the quantity of analyte present.

Charged Aerosol Detector (CAD)

Слайд 23

This is a non-optical technique and therefore is not bound by

scattering laws.
Small particles, which would be missed by ELSD, will be charged and detected by CAD.
Charging the particles produces a wider dynamic range.
In principle, the LOD should be better (not necessarily).
Produce more uniform response between compounds as it is not dependent on particle size.
Nebulization is not critical, as droplet size is irrelevant, therefore, nebulizer can set to produce smaller particles that will dry more easily.

CAD vs ELSD

Слайд 24

The technique is dependent on nebulization and evaporation stages.
It is gradient

sensitive - the response will change as solvent composition changes.
Change in solvent composition makes the technique difficult to apply to unknowns.
Highly volatile compounds will be lost through evaporation.
It is not a linear technique (but is slightly better than ELSD), so unknown quantification is difficult.
Some buffers, such as TEA, can cause problems.
Not all compounds charge easily, so LOD is still compound dependent.

CAD vs ELSD

Слайд 25

Overview of ELSD Sales
Global ELSD Market - ca. 1000-1500 units/year
PL shares

ca. 25% of the market
80% PL-ELS 2100
20% PL-ELS 1000
Main Competitors
Sedere
Alltech
ESA/Eurosep
Waters

Слайд 26

ELSD Sales by Region

Слайд 27

Competitor Overview

Слайд 28

Comparison of Competitors’ Evaporative Light Scattering Detectors

Содержание

What affects ELSD performance? NebulizationSize of droplet formed: consequently determines size

NebulizationIncrease Neb gas flow ----------- Reduces droplet sizeIncrease Liquid flow ---------------

Over the last four years, the number of ELSD manufacturers has

Alltech ELSD 800Light source: 670nm @ 5mW laser diodeDetector element: Silicon

Alltech ELSD 2000ES Light source: 650nm 30mW Laser diodeDetector element: Silicon

Alltech ELSD 3300ES Light source: 650nm 30mW Laser diodeSilicon photodiode detectorTemperature

NebulizerGas flow adjusted for solvent typeNo nebulizer temperature control EvaporationTemperature

SEDEX 85 LT-ELSDBlue light sourceExpanded software functionalityMass flow controllerRemote power-downAutomatic

Nebulizer 3.0 SLMs of gas used to nebulize solvents under

EvaporationLong coiled tube which removes large droplets by centrifugal force and

ELSD 2040 Light Source: Tungsten Halogen Lamp Detector Element:

Acquity ELSDDesigned for UPLC an analysis80Hz Data rateSharp peak shape

NebulizerSnap-on design for easy replacementTemperature controllable (% of drift tube

ELSD 400 Light source: 670nm LASER <5mWDetector element: PhotodiodeTemperature range:

Nebuliser: Thermo-split TechnologyFor difficult to evaporate mobile phases, or high

ZAM 3000 Light source: Tungsten Halogen Detector element: Photomultiplier

Nebulizer For difficult to evaporate mobile phases, or high flow

Chromachem ELSD Light source: Halogen lamp Detector element: Photomultiplier Temperature range:

Nebulizer Similar design to SEDEX 75Evaporation Similar design to SEDEX

ESA’s Charged Aerosol Detector (CAD)

How it works:The column eluent is nebulized with nitrogen, before passing

This is a non-optical technique and therefore is not bound by

The technique is dependent on nebulization and evaporation stages.It is gradient

Overview of ELSD SalesGlobal ELSD Market - ca. 1000-1500 units/yearPL shares

ELSD Sales by Region

Competitor Overview

Competitor Overview

Похожие презентации

What affects ELSD performance?
Nebulization
Size of droplet formed: consequently determines size

Nebulization
Increase Neb gas flow ----------- Reduces droplet size
Increase Liquid flow ---------------

Alltech
ELSD 800
Light source: 670nm @ 5mW laser diode
Detector element: Silicon

Alltech
ELSD 2000ES
Light source: 650nm 30mW Laser diode
Detector element: Silicon

Alltech
ELSD 3300ES
Light source: 650nm 30mW Laser diode
Silicon photodiode detector
Temperature

Nebulizer
Gas flow adjusted for solvent type
No nebulizer temperature control
Evaporation
Temperature

SEDEX 85 LT-ELSD
Blue light source
Expanded software functionality
Mass flow controller
Remote power-down
Automatic

Nebulizer
3.0 SLMs of gas used to nebulize solvents under

Evaporation
Long coiled tube which removes large droplets by centrifugal force and

ELSD 2040
Light Source: Tungsten Halogen Lamp
Detector Element:

Acquity ELSD
Designed for UPLC an analysis
80Hz Data rate
Sharp peak shape

Nebulizer
Snap-on design for easy replacement
Temperature controllable (% of drift tube

ELSD 400
Light source: 670nm LASER <5mW
Detector element: Photodiode
Temperature range:

Nebuliser: Thermo-split Technology
For difficult to evaporate mobile phases, or high

ZAM 3000
Light source: Tungsten Halogen
Detector element: Photomultiplier

Nebulizer
For difficult to evaporate mobile phases, or high flow

Chromachem ELSD
Light source: Halogen lamp
Detector element: Photomultiplier
Temperature range:

Nebulizer
Similar design to SEDEX 75
Evaporation
Similar design to SEDEX

How it works:
The column eluent is nebulized with nitrogen, before passing

The technique is dependent on nebulization and evaporation stages.
It is gradient

Overview of ELSD Sales
Global ELSD Market - ca. 1000-1500 units/year
PL shares