SNAP i.d. Workflow Value Proposition

Сентябрь 9, 2022

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SNAP i.d. Workflow Value Proposition

Содержание

2. Electro- phoresis Membrane Transfer Blocking Antibody Addition Detection Sample Prep 45 min -2 Hrs .5 -1
3. Electro- phoresis Membrane Transfer Blocking Antibody Addition Detection Sample Prep SNAP i.d. Workflow Value Proposition Western
4. Product Contents
5. SNAP i.d. Components A B C D E SNAP i.d. Base Single Blot Holder, 30/pk Double
6. Chemical duty pump (WP6111560, WP6122050) Additional Items Line Filter Millex-FA50 (SLFA05010) 1 Liter Vacuum Flask (XX1004705)
7. SNAP i.d. SKU’s “zero” Hardware Consumables Western Blotting AVIDXXXX Internal Project Codename
8. SNAP i.d. Protocol Overview: Blot Holder Assembly 1) Open Blot Holder 2) Wet Flow Distributor 3)
9. SNAP i.d. Protocol Overview: Immunodetection 1) Open Lid 2) Insert Blot Holder 3a) Add Blocking Solution
10. Feature and Benefits Summary
11. Product Position Superior Blots in a SNAP! Optimize your blots Increase the quality of your blots
12. Product position: Optimization All blots should be optimized but most researchers don’t have the time to
13. How it Works Traditional western blotting takes a variety of formats and reagent conditions to accomplish.
14. How it Works – reagent flows Gentle Rocking Reagents diffuse slowly into membrane Reagents rapidly driven
15. How it Works – reagent flows Blocking Efficient coverage of membrane which yields higher sensitivity Can
16. Compatible Blocking Reagents and Recommended Concentrations From page 8 of the SNAP i.d. User Guide
17. How it Works – reagent flows Washing Unbound Antibodies are thoroughly flushed out of the membrane
18. Standard vs. SNAP i.d. - concentrations Concentrations Blocking concentrations are limited to prevent clogging of blot
19. Concentrations: The Rule of 3.5 Block in 0.5% NFDM vs 1.0%-5.0% NFDM To prevent clogging of
20. 1° Antibody Addition & Incubation Washing 2° Antibody Addition & Incubation Washing Blocking 1 Hr 1
22. Скачать презентацию

Слайд 2

Electro-
phoresis
Membrane
Transfer
Blocking
Antibody
Addition
Detection
Sample
Prep
45 min
-2 Hrs
.5 -1 Hr
1-2.5 Hrs
1 Hr
3 Hrs
15 min
SNAP i.d. Workflow

Value Proposition

Reduces incubation times in Western Blotting
Compatible with all reagents and membranes
No directly competitive product on the market

Western Blotting Protocol

Higher quality blots with Immunodetection occurring in 30 min vs. 4 hrs

SNAP i.d.™

Слайд 3

Electro-
phoresis
Membrane
Transfer
Blocking
Antibody
Addition
Detection
Sample
Prep
SNAP i.d. Workflow Value Proposition
Western Blotting Protocol
SNAP i.d.™
Higher quality blots in

less than 30 min

5%NFDM

0.05% NFDM

Standard

Слайд 4

Product Contents

Слайд 5

SNAP i.d. Components
A
B
C
D
E
SNAP i.d. Base
Single Blot Holder, 30/pk
Double Blot Holder, 30/pk
Triple

Blot Holder, 20/pk
Antibody Collection Tray,
20/pk

7.5 cm x 8.8 cm

4.6 cm x 8.8 cm

3.2 cm x 8.8 cm

Слайд 6

Chemical duty pump
(WP6111560, WP6122050)
Additional Items
Line Filter Millex-FA50
(SLFA05010)
1 Liter Vacuum Flask
(XX1004705)
Perforated

Stopper (XX1004708)
Silicone Tubing
(XX7100004)

Vacuum source
must provide
4” Hg (135 millibar)

Слайд 7

SNAP i.d. SKU’s
“zero”
Hardware
Consumables
Western Blotting AVIDXXXX
Internal Project
Codename

Слайд 8

SNAP i.d. Protocol Overview: Blot Holder Assembly
1) Open Blot Holder
2) Wet Flow

Distributor

3) Place Blotted Membrane

4) Place Spacer

5) Close Blot Holder

6) Turn over Blot Holder

Слайд 9

SNAP i.d. Protocol Overview: Immunodetection
1) Open Lid
2) Insert Blot Holder
3a) Add Blocking

Solution
3b) Turn on Vacuum

4) Turn off Vacuum

5a) Add Primary Antibody
5b) Incubate 10 min
5c) Turn on Vacuum

6a) Add wash solution (3x)
6b) Turn off Vacuum

Repeat steps 4-6 for 2° Ab

Слайд 10

Feature and Benefits Summary

Слайд 11

Product Position
Superior Blots in a SNAP!
Optimize your blots
Increase the quality of

your blots
And do it FAST!
Results for your afternoon meeting! (Customer quote)

Superior Blots in a SNAP!

Слайд 12

Product position: Optimization
All blots should be optimized but most researchers don’t

have the time to do this tedious process.
SNAP i.d. makes optimization easy by allowing 6 blots to be processed simultaneously in only 30 minutes!
What do they typically optimize?
Antibody concentrations/dilutions
Detection reagent conditions
Target protein concentrations (more rarely)
Blocking conditions (a little secret to sensitivity)
SNAP i.d. permits fast optimization for better results.
Point out the Immunodetection handbook Western blotting section for helpful hints.

Слайд 13

How it Works
Traditional western blotting takes a variety of formats and

reagent conditions to accomplish. It’s a passive process!
SNAP i.d. actively drives reagents through the membrane to increase the quality of the blots and increase the speed of immunodetection!
It’s a combination of reagent flows and concentrations

Vs.

Standard ‘rocking’ of reagents

Actively drive reagents with vacuum flow

Слайд 14

How it Works – reagent flows
Gentle Rocking
Reagents diffuse
slowly into membrane
Reagents rapidly
driven

into membrane

Standard

Vacuum

Reagents penetrate more of the membrane 3D structure where the proteins are blotted.
Result = Increase quality of the blot in a SNAP!

Слайд 15

How it Works – reagent flows
Blocking
Efficient coverage of membrane which yields

higher sensitivity
Can use 1/10th-1/100th less concentrated blocking solution to minimize overblocking
Actively driven vacuum flow coats inner surfaces of membrane in 20 sec

GAPDH

5%NFDM

0.5% NFDM

0.1% NFDM

0.05% NFDM

Standard

1 2 3 4 5 6 7 8

Слайд 16

Compatible Blocking Reagents and Recommended Concentrations
From page 8 of the SNAP

i.d. User Guide

Слайд 17

How it Works – reagent flows
Washing
Unbound Antibodies are thoroughly flushed out

of the membrane
Yields lower backgrounds ?which may yield higher Sensitivity

GAPDH

5%NFDM

0.5% NFDM

0.1% NFDM

0.05% NFDM

Standard

1 2 3 4 5 6 7 8

Слайд 18

Standard vs. SNAP i.d. - concentrations
Concentrations
Blocking concentrations are limited to prevent

clogging of blot holder
Antibody concentrations are increased to speed up reaction kinetics

Слайд 19

Concentrations: The Rule of 3.5
Block in 0.5% NFDM vs 1.0%-5.0% NFDM
To

prevent clogging of flow distributor
To minimize overblocking and maximize sensitivity

Rule of 3.5

1° & 2° Ab concentration
and volume

Blocking concentration

Use 1/3 the volume of Ab at 3x concentration
Concentration: Drives Ab incubation kinetics faster
Dead volume: Minimizes Ab consumption

Слайд 20

1° Antibody
Addition &
Incubation
Washing
2° Antibody
Addition &
Incubation
Washing
Blocking
1 Hr
1 Hr-overnight
15 min
1 Hr
15 min
How it

Works: Time savings

Western Blotting Protocol

20 sec

10 min

1 min

10 min

1 min

Standard

Electro-
phoresis

Membrane
Transfer

Blocking

Antibody
Addition

Detection

Sample
Prep

SNAP i.d.™

22 min

4 Hrs

Vs.

SNAP i.d. Workflow Value Proposition

Содержание

Electro-phoresisMembraneTransferBlockingAntibodyAdditionDetectionSamplePrep45 min-2 Hrs.5 -1 Hr1-2.5 Hrs1 Hr3 Hrs15 minSNAP i.d. Workflow

Electro-phoresisMembraneTransferBlockingAntibodyAdditionDetectionSamplePrepSNAP i.d. Workflow Value PropositionWestern Blotting ProtocolSNAP i.d.™Higher quality blots in

Product Contents

SNAP i.d. ComponentsABCDESNAP i.d. BaseSingle Blot Holder, 30/pkDouble Blot Holder, 30/pkTriple

Chemical duty pump(WP6111560, WP6122050)Additional ItemsLine Filter Millex-FA50 (SLFA05010)1 Liter Vacuum Flask(XX1004705)Perforated

SNAP i.d. SKU’s“zero”HardwareConsumablesWestern Blotting AVIDXXXXInternal ProjectCodename

SNAP i.d. Protocol Overview: Blot Holder Assembly1) Open Blot Holder2) Wet Flow

SNAP i.d. Protocol Overview: Immunodetection1) Open Lid2) Insert Blot Holder3a) Add Blocking

Feature and Benefits Summary

Product PositionSuperior Blots in a SNAP!Optimize your blotsIncrease the quality of

Product position: OptimizationAll blots should be optimized but most researchers don’t

How it WorksTraditional western blotting takes a variety of formats and

How it Works – reagent flowsGentle RockingReagents diffuseslowly into membraneReagents rapidlydriven

How it Works – reagent flowsBlockingEfficient coverage of membrane which yields

Compatible Blocking Reagents and Recommended ConcentrationsFrom page 8 of the SNAP

How it Works – reagent flowsWashingUnbound Antibodies are thoroughly flushed out

Standard vs. SNAP i.d. - concentrationsConcentrationsBlocking concentrations are limited to prevent

Concentrations: The Rule of 3.5Block in 0.5% NFDM vs 1.0%-5.0% NFDMTo

1° AntibodyAddition &IncubationWashing2° AntibodyAddition &IncubationWashingBlocking1 Hr1 Hr-overnight15 min1 Hr15 minHow it

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Electro-
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Membrane
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Blocking
Antibody
Addition
Detection
Sample
Prep
45 min
-2 Hrs
.5 -1 Hr
1-2.5 Hrs
1 Hr
3 Hrs
15 min
SNAP i.d. Workflow

Electro-
phoresis
Membrane
Transfer
Blocking
Antibody
Addition
Detection
Sample
Prep
SNAP i.d. Workflow Value Proposition
Western Blotting Protocol
SNAP i.d.™
Higher quality blots in

SNAP i.d. Components
A
B
C
D
E
SNAP i.d. Base
Single Blot Holder, 30/pk
Double Blot Holder, 30/pk
Triple

Chemical duty pump
(WP6111560, WP6122050)
Additional Items
Line Filter Millex-FA50
(SLFA05010)
1 Liter Vacuum Flask
(XX1004705)
Perforated

SNAP i.d. SKU’s
“zero”
Hardware
Consumables
Western Blotting AVIDXXXX
Internal Project
Codename

SNAP i.d. Protocol Overview: Blot Holder Assembly
1) Open Blot Holder
2) Wet Flow

SNAP i.d. Protocol Overview: Immunodetection
1) Open Lid
2) Insert Blot Holder
3a) Add Blocking

Product Position
Superior Blots in a SNAP!
Optimize your blots
Increase the quality of

Product position: Optimization
All blots should be optimized but most researchers don’t

How it Works
Traditional western blotting takes a variety of formats and

How it Works – reagent flows
Gentle Rocking
Reagents diffuse
slowly into membrane
Reagents rapidly
driven

How it Works – reagent flows
Blocking
Efficient coverage of membrane which yields

Compatible Blocking Reagents and Recommended Concentrations
From page 8 of the SNAP

How it Works – reagent flows
Washing
Unbound Antibodies are thoroughly flushed out

Standard vs. SNAP i.d. - concentrations
Concentrations
Blocking concentrations are limited to prevent

Concentrations: The Rule of 3.5
Block in 0.5% NFDM vs 1.0%-5.0% NFDM
To

1° Antibody
Addition &
Incubation
Washing
2° Antibody
Addition &
Incubation
Washing
Blocking
1 Hr
1 Hr-overnight
15 min
1 Hr
15 min
How it