Lab Safety (pp7-9 of lab supplement)

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Lab Safety (pp7-9 of lab supplement)

Содержание

3. Emergency procedures Where is our nearest exit? Where do we go if we have to evacuate?
4. Workplace Hazardous Materials Information System 3 parts to the system… Education Labels SDS sheets
5. In regular lab activities, students are responsible for: Reading labels in the lab Identifying the hazards
6. Workplace label = much simpler; most often what you will see in the lab Name of
7. Know the symbols and what they mean! Which ones do you see in the lab today?
8. Introduction to Microscopes BIOL 104: Lab 1
9. Goals for today: Compare longitudinal and cross sections Compare four types of microscopes Demonstrate correct use
10. Function of Microscopes Create a magnified image of a specimen while maintaining resolution Magnification: create an
11. Increased magnification with no change in resolution Increased magnification with increased resolution
12. Electron Microscopes Use electron beam to create image Advantages High magnification High resolution Drawbacks Expensive Require
13. Transmission EM 2D image Specimen has to be sectioned (=sliced very thin) Scanning EM Can view
14. Preparing specimens may involve sectioning Longitudinal section Cross section
15. Light microscopes Compound Microscope Stereo Microscope Specimen goes here Specimen goes here
16. Compare types of light microscope: Other comparisons to consider: Is the image 3D or 2D? Is
17. COMPOUND MICROSCOPES
18. Scanning Low High Magnification
19. Working Distance: Space between specimen and magnifying lens ALWAYS START AT LOWEST MAGNIFICATION As you increase
20. Increasing magnification Field of view Field of view
21. Field of View How much of the specimen is visible = field of view Changes as
22. Depth of field Compound microscope gives 2D image Need to refocus to see top and bottom
23. Today’s to do list: Complete all activities in the “Microscopy” lab in your lab manual You
25. Скачать презентацию

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Emergency procedures
Where is our nearest exit?
Where do we go if we

have to evacuate?

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Workplace Hazardous Materials Information System
3 parts to the system…
Education
Labels
SDS sheets

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In regular lab activities, students are responsible for:
Reading labels in

the lab
Identifying the hazards associated with the labels
Handling chemicals according to the warnings on the label
Following instructions given in the lab regarding disposal and clean-up.

Be familiar with the WHMIS brochure in your Lab Supplement:
Labels: Workplace vs Supplier Label
Symbols for safety equipment
Hazard symbols (pictograms)
SDS (Safety Data Sheet)

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Workplace label = much simpler; most often what you will see

in the lab

Name of the product
Safe handling, may include pictograms
Reference to SDS

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Know the symbols and what they mean! Which ones do you see

in the lab today?

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Introduction to Microscopes
BIOL 104: Lab 1

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Goals for today:
Compare longitudinal and cross sections
Compare four types of microscopes
Demonstrate

correct use of stereomicroscope and compound microscope
Be able to estimate size of objects with microscope
Practice making wet mount slides and viewing life specimens
Answer all questions in lab manual

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Function of Microscopes
Create a magnified image of a specimen while maintaining

resolution
Magnification: create an image that appears larger
Resolution: ability to distinguish two objects/points from one another

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Increased magnification with no change in resolution
Increased magnification with increased resolution

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Electron Microscopes
Use electron beam to create image
Advantages
High magnification
High resolution
Drawbacks
Expensive
Require

extensive and specialized prep of specimens

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Transmission EM
2D image
Specimen has to be sectioned (=sliced very thin)
Scanning EM
Can

view whole specimens
3D image (surface of specimen)

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Preparing specimens may involve sectioning
Longitudinal section
Cross section

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Light microscopes
Compound Microscope
Stereo Microscope
Specimen goes here
Specimen goes here

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Compare types of light microscope:
Other comparisons to consider:
Is the image 3D

or 2D? Is it inverted or same as naked eye? Surface of specimen or internal feature visible…

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COMPOUND MICROSCOPES

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Scanning
Low
High
Magnification

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Working Distance: Space between specimen and magnifying lens
ALWAYS START AT LOWEST

MAGNIFICATION
As you increase magnification, working distance decreases
Important to “Focus First”: be in focus before you change magnification!

4 x

10 x

40 x

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Increasing magnification
Field of view
Field of view

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Field of View
How much of the specimen is visible = field

of view
Changes as you magnify
Page 9: Measure field of view with ruler (use the ruler like a slide!) and calculate field of view at other magnifications

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Depth of field
Compound microscope gives 2D image
Need to refocus to see

top and bottom of an object
Use “crossed threads” prepared slide to see this
Are you moving the slide up or down?
Which will you see first, the top or the bottom?

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Today’s to do list:
Complete all activities in the “Microscopy” lab in

your lab manual
You can do Stereomicroscope later if stations are busy-everyone must use their own compound microscope
Please ask for help if you do not understand the instructions!
Answer questions on the last page
After you are cleaned up, check in with Eunice to complete the post-lab quiz

Lab Safety (pp7-9 of lab supplement)

Содержание

Emergency proceduresWhere is our nearest exit?Where do we go if we

Workplace Hazardous Materials Information System 3 parts to the system… Education Labels SDS sheets

In regular lab activities, students are responsible for: Reading labels in

Workplace label = much simpler; most often what you will see

Know the symbols and what they mean! Which ones do you see

Introduction to MicroscopesBIOL 104: Lab 1

Goals for today:Compare longitudinal and cross sectionsCompare four types of microscopesDemonstrate

Function of MicroscopesCreate a magnified image of a specimen while maintaining

Increased magnification with no change in resolutionIncreased magnification with increased resolution

Electron MicroscopesUse electron beam to create imageAdvantages High magnification High resolutionDrawbacksExpensiveRequire

Transmission EM2D imageSpecimen has to be sectioned (=sliced very thin)Scanning EMCan

Preparing specimens may involve sectioningLongitudinal sectionCross section

Light microscopesCompound MicroscopeStereo MicroscopeSpecimen goes hereSpecimen goes here

Compare types of light microscope:Other comparisons to consider:Is the image 3D