Transpiration in plants. The release of water from plant leaves

Содержание

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TRANSPIRATION Transpiration is the loss of water from a plant by

TRANSPIRATION

Transpiration is the loss of water from a plant by evaporation
Water

can only evaporate from the plant if the water potential is lower in the air surrounding the plant
Most transpiration occurs via the leaves
Most of this transpiration is via the stomata.
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HOW TRANSPIRATION IS MEASURED A Simple Potometer Air tight seals Plastic

HOW TRANSPIRATION IS MEASURED

A Simple Potometer

Air tight seals

Plastic tubing

Graduated scale

Capillary tube

Leafy

shoot cut under water

Water evaporates from the plant

Movement of meniscus is measured over time

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HOW TRANSPIRATION IS MEASURED 1’’’’’’’’2’’’’’’’’3’’’’’’’’4’’’’’’’’5’’’’’’’’6’’’’’’’’7’’’’’’’’8’’’’’’’’9’’’’’’’’10’’’’’’’’11’’’’’’’’12’’’’’’’’13’’’’ The rate of water loss from

HOW TRANSPIRATION IS MEASURED

1’’’’’’’’2’’’’’’’’3’’’’’’’’4’’’’’’’’5’’’’’’’’6’’’’’’’’7’’’’’’’’8’’’’’’’’9’’’’’’’’10’’’’’’’’11’’’’’’’’12’’’’’’’’13’’’’

The rate of water loss from the shoot

can be measured under different environmental conditions

volume of water taken up in given time

Limitations

measures water uptake

cutting plant shoot may damage plant

plant has no roots so no resistance to water being pulled up

Water is pulled up through the plant

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ENVIRONMENTAL FACTORS AFFECTING TRANSPIRATION Relative humidity:- air inside leaf is saturated

ENVIRONMENTAL FACTORS AFFECTING TRANSPIRATION

Relative humidity:- air inside leaf is saturated

(RH=100%). The lower the relative humidity outside the leaf the faster the rate of transpiration as the Ψ gradient is steeper
Air Movement:- increase air movement increases the rate of transpiration as it moves the saturated air from around the leaf so the Ψ gradient is steeper.
Temperature:- increase in temperature increases the rate of transpiration as higher temperature
Provides the latent heat of vaporisation
Increases the kinetic energy so faster diffusion
Warms the air so lowers the Ψ of the air, so Ψ gradient is steeper
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4. Atmospheric pressure:- decrease in atmospheric pressure increases the rate of

4. Atmospheric pressure:- decrease in atmospheric pressure increases the rate of

transpiration.
5. Water supply:- transpiration rate is lower if there is little water available as transpiration depends on the mesophyll cell walls being wet (dry cell walls have a lower Ψ). When cells are flaccid the stomata close.
6. Light intensity :- greater light intensity increases the rate of transpiration because it causes the stomata to open, so increasing evaporation through the stomata.
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THE EFFECT OF WIND SPEED ON THE RATE OF TRANSPIRATION Stomata

THE EFFECT OF WIND SPEED ON THE RATE OF TRANSPIRATION

Stomata diameter/µm

10

20

Stomatal

transpiration rate
/ gcm-2s-1

In still air closing the stomata is less effective in controlling the transpiration rate

moving air

still air

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MOVING AIR REMOVES THE BOUNDARY LAYER OF WATER VAPOUR FROM THE

MOVING AIR REMOVES THE BOUNDARY LAYER OF WATER VAPOUR FROM THE

LEAF

Boundary layer

Saturated air accumulates around leaf

Still air

Moving air

Lower Ψ

Water vapour is removed from the leaf surface

the Ψ gradient is increased, so faster rate of water evaporation via the stomata

cross section through a leaf

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MOVEMENT OF WATER THROUGH THE STOMATA H2O Diffusion shells Water moves

MOVEMENT OF WATER THROUGH THE STOMATA

H2O

Diffusion shells

Water moves from a higher

(less negative) to a lower (more negative) water potential
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INCREASE IN STOMATAL FREQUENCY INCREASES THE RATE OF TRANSPIRATION Boundary layer

INCREASE IN STOMATAL FREQUENCY INCREASES THE RATE OF TRANSPIRATION

Boundary layer

If the

distance between the stomata is less than 10 X the pore diameter the diffusion shells overlap

So increasing the number of stomata per unit area will have no further effect on transpiration

stoma

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WILTING If water lost by transpiration is greater than water uptake

WILTING

If water lost by transpiration is greater than water uptake via

the roots the plant cells become flaccid and the plant wilts.

When the guard cells are flaccid the stomata close

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LEAF SECTION The upper epidermis has no stomata The lower epidermis

LEAF SECTION

The upper epidermis has no stomata

The lower epidermis has stomata.

The

guard cells control the opening and closing of the stomata
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SURFACE VIEW OF LEAF EPIDERMIS SHOWING THE GUARD CELLS WHICH ARE FLACCID AND THE STOMA CLOSED.

SURFACE VIEW OF LEAF EPIDERMIS SHOWING THE GUARD CELLS WHICH ARE

FLACCID AND THE STOMA CLOSED.
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THE GUARD CELLS CONTROL THE OPENING AND CLOSING OF THE STOMATA

THE GUARD CELLS CONTROL THE OPENING AND CLOSING OF THE STOMATA


Thin outer wall

Guard cells flaccid

Stoma closed

Stoma open

Guard cells turgid

Thick inner wall

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REGULATING STOMATAL OPENING:-THE POTASSIUM ION PUMP HYPOTHESIS Guard cells flaccid Stoma

REGULATING STOMATAL OPENING:-THE POTASSIUM ION PUMP HYPOTHESIS

Guard cells flaccid

Stoma closed

K+

K+

K+

K+

K+

K+

K+

K+

K+

K+

K+

K+

K+

ions have the same concentration in guard cells and epidermal cells

Light activates K+ pumps which actively transport K+ from the epidermal cells into the guard cells

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REGULATING STOMATAL OPENING:-THE POTASSIUM ION PUMP HYPOTHESIS K+ K+ K+ K+

REGULATING STOMATAL OPENING:-THE POTASSIUM ION PUMP HYPOTHESIS

K+

K+

K+

K+

K+

K+

K+

K+

K+

K+

K+

K+

Increased concentration of K+

in guard cells

Lowers the Ψ in the guard cells

Water moves in by osmosis, down Ψ gradient

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Stoma open Guard cells turgid K+ K+ K+ K+ K+ K+

Stoma open

Guard cells turgid

K+

K+

K+

K+

K+

K+

K+

K+

K+

K+

K+

K+

Increased concentration of K+ in guard cells

Lowers the

Ψ in the guard cells

Water moves in by osmosis, down Ψ gradient

H2O

H2O

H2O

H2O

H2O

H2O

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24H CYCLE OF STOMATAL OPENING AND CLOSING 18.00 12.00 15.00 21.00

24H CYCLE OF STOMATAL OPENING AND CLOSING

18.00

12.00

15.00

21.00

24.00

3.00

06.00

09.00

Why is this cycle an

advantage to most plants?