Fig. 1. Replication cycle of rotaviruses in Cytoplasm of the cell
The
different steps in the replication cycle of the virus are indicated by numbers— 1: attachment of the virion to the cell surface;
2: penetration and uncoating of the virus particle to yield DLPs;
3: primary transcription of the genomic dsRNA;
4: synthesis of viral proteins;
5: assembly of core RIs and negative strand RNA synthesis;
6: assembly of double-layered RIs;
7: secondary transcription from double-layered RIs;
8: secondary, enhanced synthesis of viral proteins;
9: secondary, increased assembly of core RIs and negative strand RNA synthesis;
10: secondary, increased assembly of double-layered RIs;
11: budding of double-layered RIs through the membrane of the endoplasmic reticulum (ER), and acquisition of a membrane envelope;
12: loss of the membrane envelope and generation of mature triple-layered virions.
Carlos F. Arias, 2004,
RNA silencing of rotavirus gene expression
Virus Research 102 (2004) 43–51