Disease of Honeybees

Август 12, 2022

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Биология
Disease of Honeybees

Содержание

2. Introduction Honeybees are attacked both at brood and adult stages by microorganisms. The disease of honeybees
3. TYPES OF DISEASES : Protozoan -Nosema, Amoebic Bacterial- American and Europian Foul brood Fungal -Chalk brood,
4. Brood diseases American foul brood European foul brood Fungal brood diseases Chalk brood Stone brood Viral
5. American foul disease The most destructive microbial disease in temperate and sub-tropical region all over the
6. Diagnostic procedure: The simple test for AFD is the “Stretch test” A match stick or tooth
7. Feeding streptomycine in sugar syrup @ 0.05-0.15g/litre. Dust Terramycin(TM50) in powdered sugar (1.20) @ 4 tea
8. European foul Brood In India, except Maharastra, EFB has not been recorded so for in Apis
9. The color of the larvae changes from shiny white to pale yellow and then brown, as
10. Fungal Brood Diseases Chalk brood Cause: causative agent is Ascophaer apis It is a heterothallic fungus
11. Treatment: Equipment's should be sterilized using formalin or carbolic acid. 0. 7% of thymol has been
12. Stone Brood Cause: it is caused by Aspergillus niger, Aspergillus fumigates, Aspergillus flavus. Symptoms: Mummification of
13. Viral Diseases So far, the world over, 18 viruses have been found to infect honeybees. Thai
14. Kashmir bee virus Kashmir bee virus is related to the preceding viruses. Recently discovered, it is
15. Control measurements: use antibiotics such as Rifampin, Leavamisol, Amentidine along with vitamin B complex fed to
16. Adult Bee Diseases: The diseases of adult bees are caused by protozoa which are single celled
17. Disease of Adult Bees Apis Iridescent Virus Symptoms: Reduced egg laying / brood rearing. Bees become
18. Management of Viral Diseases: For viral pathogens, there is no chemical control. Affected colonies should be
19. Nosema Disease: This disease is caused by Nosema apis Zander. It is disease of adult bees.
20. Symptoms Bees start foraging at younger age. Bees feel fatigued, are less able to fly and
21. Mid intestine is swollen and if dissected, shows dull greyish white contents. Bees soil the hive
22. ACAROSIS (The Honey Bee Tracheal Mite) CAUSAL AGENT- honey bee tracheal mite, Acarapis woodi, a small
23. PLACE OF INFECTION: Trachea and body fluid MANAGEMENT:- Use of grease patties ( typically made from
24. VARROASIS (The Varroa Mite) CAUSAL AGENT: Asiatic varroa mite, Varroa destructor. Symptoms: Varroa reproduce on honey
25. PLACE OF INFECTION: Body and body fluid i.e. haemolymph MANAGEMENT: Apivar: Apivar is effective against varroa
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Introduction
Honeybees are attacked both at brood and adult stages by

microorganisms.
The disease of honeybees are divided into brood disease and adult disease.

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TYPES OF DISEASES :
Protozoan -Nosema, Amoebic
Bacterial- American and Europian Foul

brood
Fungal -Chalk brood, Stone brood
Viral -Thai sac brood, Acute and Chronic bee paralysis
Diseases caused by Mites viz. Acarosis (Tracheal mite) and Varroasis (Varroa mite)
Disorder- Colony Collapse Disorders (CCD)

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Brood diseases
American foul brood
European foul brood
Fungal brood diseases

Chalk brood
Stone brood
Viral diseases
Thai sac brood
Nosema disease

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American foul disease
The most destructive microbial disease in temperate and

sub-tropical region all over the world.
Cause:
spore forming bacterium, Bacillus Larvae
Only affect brood
Symptoms:
Initial stage, isolated capped cells from which brood has not emerged can be seen on the comb.
Caps of these brood cells are darker than the caps of healthy cells..
The infected brood die at prepupal or late larval stage.
The dead brood is dull white in color, but gradually changes to light brown, coffee brown and finally dark brown or almost black.

Dead Pupae

Irregular and sunken brood

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Diagnostic procedure:
The simple test for AFD is the “Stretch test”
A match

stick or tooth stick is inserted into the body of the decayed larva and then, gently and slowly, withdrawn.
If the disease is present, the dead larval content adhere to the tip of the stick, stretching up to 2.5 cm before breaking and snapping back in somewhat elastic way.
Treatment:
Feeding of sodium sulphathiazole @ 0.1g/litre in sugar surup
Feeding oxytetracycline(Terramycin) 0.25 to 0.4g in 5 litre sugar syrup.

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Feeding streptomycine in sugar syrup @ 0.05-0.15g/litre.
Dust Terramycin(TM50) in powdered sugar

(1.20) @ 4 tea spoon full on top bars of the brood frames
Chemotherapy has no effect on spores that contaminate the equipment.
Chemotherapy is not advisable for low disease indication.

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European foul Brood
In India, except Maharastra, EFB has not been recorded

so for in Apis Mellifera colonies.
Cause:
Pathogenic gram negative bacterium Melissococcus pluton
Symptoms:
Honeybee larvae killed by EFB are in younger stages than those killed by AFB.
The diseased larvae die when they are 4 to 5 days old, or in the coiled stage.

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The color of the larvae changes from shiny white to pale

yellow and then brown, as it decays.
Another symptom of EFB is that most of the affected larvae die before their cells are capped.
Treatment:
Not required if the infection is low.
Sterilization of combs and honey could be done with formaldehyde and acetic acid.
0.5 or 1 g oxytetracycline (tetramycin) dissolved in 500 ml of concentrated sucrose syrup sprinkled on bee clusters gave good results.

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Fungal Brood Diseases
Chalk brood
Cause: causative agent is Ascophaer apis
It

is a heterothallic fungus
Colonies rarely die from disease, but in some cause honey yielda may be reduced.
Symptoms:
It affects honeybee larvae or brood.
Chalk brood is usually more common on the edges of the brood chamber
It is most common in drone brood.
Initially, the dead larvae are swollen to the size of the cell and covered with the whitish mycelia of the fungus.
Subsequently, the dead larvae become mummified, hard, shrunken and chalk like in appearance.

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Treatment:
Equipment's should be sterilized using formalin or carbolic acid.
0. 7% of

thymol has been reported to prevent the growth of fungus.
Amphotericin B, sorbic acid and sodium propionate fed to bees in pollen-sugar patties controlled infection within 7 days without affecting bees.
NistapiR controlled more than 98% of the infection in affected colonies.
Increasing ventilation
Removing “mummies” from bottom board and around the entrance.
Destroying combs containing large numbers of mummies.

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Stone Brood
Cause: it is caused by Aspergillus niger, Aspergillus fumigates,

Aspergillus flavus.
Symptoms:
Mummification of brood
Larvae and pupae that are infected with A.flavus turn green in contrast to white or black in chalk brood.
The green growth is powdery and be readily ssen with unaided eye.
Fungus spores are found most abundantly near the head of the affected larvae and pupae.
Stone brood diseased larvae are solid mummies and not sponge like as in chalk brood.

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Viral Diseases
So far, the world over, 18 viruses have been

found to infect honeybees.
Thai sac brood virus and Apis iridescent virus have been found to cause heavy losses to bee industry in India.
Thai sac brood
Cause : it is caused by a virus (TSBV) which primarily infects the larvae of Apis cerana and is closely related to sac brood virus(SBV) which infects the Apis mellifera L., but the two are not identical.
Symptoms:
This results in the death of the larva in the uncapped cells.
Diseased colonies show irregularly capped brood with sunken and faded caps.
The head of the dead larva is turned up partly across the cell opening.
Infected larva turn pale yellow and finally brownish when dead.

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Kashmir bee virus
Kashmir bee virus is related to the preceding viruses. Recently discovered,

it is currently only positively identifiable by a laboratory test. Little is known about it yet.
Black queen cell virus
Black queen cell virus causes the queen larva to turn black and die. It is thought to be associated with Nosema.
Cloudy wing virus
Cloudy wing virus is a little-studied, small, icosahedral virus commonly found in honey bees, especially in collapsing colonies infested byVarroa destructor, providing circumstantial evidence that the mite may act as a vector.

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Control measurements: use antibiotics such as Rifampin, Leavamisol, Amentidine along with

vitamin B complex fed to honeybee colonies @ 250mg/4 lit of sugay syrup at weekly intervals.
Avoiding overcrowding.

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Adult Bee Diseases:
The diseases of adult bees are caused by protozoa

which are single celled animals and form spores or cysts.
They multiply by sexual or asexual methods.
Their infection reduces vitality of bees, and shortens their life and fecundity.
Protozoan is perfect parasites as they do not kill the host immediately.
These diseases are difficult to diagnose, though inability to fly, unhooked wings and dysentery can be treated as general symptoms of an unhealthy bee.
Microscopic examination is often necessary for a definite diagnosis.

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Disease of Adult Bees
Apis Iridescent Virus
Symptoms:
Reduced egg laying / brood rearing.
Bees

become sluggish and make cluster.
The infected bees crawl on the ground.
The affected bees cease foraging, reduce honey collection, and the colony face starvation and starts dwindling.
Illuminated body tissue can be seen under microscope look bluish / greenish.
Spread: Disease is caused by iridovirus. Its infection is serious during hot / dearth seasons. It is transmitted by nurse bees through glandular secretion (food).

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Management of Viral Diseases:
For viral pathogens, there is no chemical control.
Affected

colonies should be isolated beyond their flight range.
Provide proper ventilation to reduce humidity.
Cage the queen for a week and then requeen.
Use sterilized equipments / combs.
Check robbing, drifting and swarming.
Provide supplement feeding.
Undertake selective breeding for natural resistance.

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Nosema Disease:
This disease is caused by Nosema apis Zander.
It is

disease of adult bees.
It parasitizes all the castes.
Their spores germinate in the ventriculus of the host.
Pathogen multiplies in epithilial cells
One affected bee may contain 180 million spores.
Infection spreads through ingestion of fecal matter with contaminated food.

Hind gut is inflamed by protozoa

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Symptoms
Bees start foraging at younger age.
Bees feel fatigued, are less able

to fly and fall down during their return journey.
Abdomen is distended with fecal matter.
Body hairs are lost and bees become shiny.

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Mid intestine is swollen and if dissected, shows dull greyish white

contents.
Bees soil the hive entrance.
Management:
Provide fresh running water. Drain off stagnant water from the apiary.
Provide upward ventilation to reduce humidity.
Feed fumagillin in concentrated syrup. It inhibits DNA replication of the pathogen.
Disinfect the empty hives with ethylene oxide or acetic acid fumigation @ 120 ml / hive.

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ACAROSIS (The Honey Bee Tracheal Mite)
CAUSAL AGENT- honey bee tracheal mite,

Acarapis woodi, a small parasitic mite. It affects mostly the trachea and body fluid.
Nature of damage:-
It infects worker, drone and queen honey bees. mites live and reproduce in trachea.
They pierce the tracheal tube walls & feed on the hemolymph of the bees.
feeding on blood & depositing their faeces in the passage.
STAGE OF INFECTION:- adult

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PLACE OF INFECTION: Trachea and body fluid
MANAGEMENT:-
Use of grease patties (

typically made from 1 part vegetable shortenings mixed with 3-4 parts powdered sugar) placed on top bars of the hive. Menthol allowed to vaporize from crystal form or mixed into the grease patties.
Use of resistant hybrid bees known as Buckfast bee, developed by Brother Adam at the Buckfast Abbey.
Cotton soaked in Methyl salcilate and placed under the hive in flat perforated lid.
Destruction of affected colony.
Smoke fumigation with Chlorobenzilate
Time of Treatment: Spring and early summer

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VARROASIS (The Varroa Mite)
CAUSAL AGENT: Asiatic varroa mite, Varroa destructor.
Symptoms:
Varroa reproduce

on honey bee pupae and feed on bee hemolymph.
Varroa are also known to carry and vector bee viruses that are particularly damaging to the bees.
Varroa infestations can cause irreversible damage to honey bees that can lead to honey bee colony losses.
STAGE OF INFECTION: Larval

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PLACE OF INFECTION: Body and body fluid i.e. haemolymph
MANAGEMENT:
Apivar: Apivar is

effective against varroa mites, Apistan-resistant varroa mites, and Checkmite+ resistant varroa mites.
Using 65% formic acid
Mite Away Quick Strip (MAQS): MAQS is a 7-day, single application mite control product registered for use against varroa and tracheal mites.
Time of control: Spring and early summer

Disease of Honeybees

Содержание

Introduction Honeybees are attacked both at brood and adult stages by

TYPES OF DISEASES :Protozoan -Nosema, Amoebic Bacterial- American and Europian Foul

Brood diseases American foul brood European foul broodFungal brood diseases

American foul disease The most destructive microbial disease in temperate and

Diagnostic procedure:The simple test for AFD is the “Stretch test”A match

Feeding streptomycine in sugar syrup @ 0.05-0.15g/litre.Dust Terramycin(TM50) in powdered sugar

European foul BroodIn India, except Maharastra, EFB has not been recorded

The color of the larvae changes from shiny white to pale

Fungal Brood Diseases Chalk broodCause: causative agent is Ascophaer apis It

Treatment:Equipment's should be sterilized using formalin or carbolic acid.0. 7% of

Stone Brood Cause: it is caused by Aspergillus niger, Aspergillus fumigates,

Viral Diseases So far, the world over, 18 viruses have been

Kashmir bee virusKashmir bee virus is related to the preceding viruses. Recently discovered,

Control measurements: use antibiotics such as Rifampin, Leavamisol, Amentidine along with

Adult Bee Diseases:The diseases of adult bees are caused by protozoa

Disease of Adult Bees Apis Iridescent VirusSymptoms:Reduced egg laying / brood rearing.Bees

Management of Viral Diseases:For viral pathogens, there is no chemical control.Affected

Nosema Disease: This disease is caused by Nosema apis Zander. It is

SymptomsBees start foraging at younger age.Bees feel fatigued, are less able