Mendelelian Genetics

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Gregor Mendel (1822-1884) Responsible for the Laws governing Inheritance of Traits. copyright cmassengale

Gregor Mendel (1822-1884)

Responsible for the Laws governing Inheritance of Traits.

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Gregor Johann Mendel Austrian monk Studied the inheritance of traits in

Gregor Johann Mendel

Austrian monk
Studied the inheritance of traits in pea plants.
Developed

the laws of inheritance.
Mendel's work was not recognized until the turn of the 20th century.

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Gregor Johann Mendel Between 1856 and 1863, Mendel cultivated and tested

Gregor Johann Mendel

Between 1856 and 1863, Mendel cultivated and tested some

28,000 pea plants.
He found that the plants' offspring retained traits of the parents.
Called the “Father of Genetics“.

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Site of Gregor Mendel’s experimental garden in the Czech Republic. copyright cmassengale

Site of Gregor Mendel’s experimental garden in the Czech Republic.

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Mendel stated that physical traits are inherited as “particles”. Mendel did

Mendel stated that physical traits are inherited as “particles”.
Mendel did not

know that the “particles” were actually Chromosomes & DNA.

Particulate Inheritance

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Genetic Terminology Trait - any characteristic that can be passed from

Genetic Terminology

Trait - any characteristic that can be passed from parent

to offspring.
Heredity - passing of traits from parent to offspring.
Genetics - study of heredity.

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Types of Genetic Crosses Monohybrid cross - cross involving a single

Types of Genetic Crosses

Monohybrid cross - cross involving a single trait e.g.

flower color.
Dihybrid cross - cross involving two traits. e.g. flower color & plant height.

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Punnett Square Used to help solve genetics problems copyright cmassengale

Punnett Square

Used to help solve genetics problems

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Designers - “Genes” Alleles - two forms of a gene (dominant

Designers - “Genes”

Alleles - two forms of a gene (dominant &

recessive).
Dominant - stronger of two genes expressed in the hybrid; represented by a capital letter (R).
Recessive - gene that shows up less often in a cross; represented by a lowercase letter (r).

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Genotype - gene combination for a trait. (e.g. RR, Rr, rr)

Genotype - gene combination for a trait. (e.g. RR, Rr, rr)


Phenotype - the physical feature resulting from a genotype. (e.g. red, white)

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Genotype & Phenotype in Flowers Genotype of alleles: R = red

Genotype & Phenotype in Flowers

Genotype of alleles: R = red flower r =

yellow flower
All genes occur in pairs, so 2 alleles affect a characteristic
Possible combinations are:

Genotypes RR Rr rr
Phenotypes RED RED YELLOW

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Genotypes Homozygous genotype - gene combination involving 2 dominant or 2

Genotypes

Homozygous genotype - gene combination involving 2 dominant or 2 recessive

genes (e.g. RR or rr); also called pure. 
Heterozygous genotype - gene combination of one dominant & one recessive allele. (e.g. Rr); also called hybrid.

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Genes and Environment Determine Characteristics copyright cmassengale

Genes and Environment Determine Characteristics

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Mendel’s Pea Plant Experiments copyright cmassengale

Mendel’s Pea Plant Experiments

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Why peas, Pisum sativum? Can be grown in a small area.

Why peas, Pisum sativum?

Can be grown in a small area.
Produce

lots of offspring.
Produce pure plants when allowed to self-pollinate. several generations
Can be artificially cross-pollinated.

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Reproduction in Flowering Plants Pollen contains sperm Produced by the stamen.

Reproduction in Flowering Plants

Pollen contains sperm
Produced by the stamen.
Ovary contains eggs
found

inside the flower.

Pollen carries sperm to the eggs for fertilization.
Self-fertilization can occur in the same flower.
Cross-fertilization can occur between flowers.

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Mendel’s Experimental Methods Mendel hand-pollinated flowers using a paintbrush He could

Mendel’s Experimental Methods

Mendel hand-pollinated flowers using a paintbrush
He could snip the

stamens to prevent self-pollination.
Covered each flower with a cloth bag.
He traced traits through the several generations.


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How Mendel Began Mendel produced pure strains by allowing the plants

How Mendel Began

Mendel produced pure strains by allowing the plants to

self-pollinate for several generations.

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EightTraits of Pea Plant Seed shape --- Round (R) or Wrinkled

EightTraits of Pea Plant

Seed shape --- Round (R) or Wrinkled (r)
Seed

Color ---- Yellow (Y) or Green (y)
Pod Shape --- Smooth (S) or Wrinkled (s)
Pod Color --- Green (G) or Yellow (g)
Seed Coat Color --- Gray (G) or White (g)
Flower position---Axial (A) or Terminal (a)
Plant Height --- Tall (T) or Short (t)
Flower color --- Purple (P) or White (p)

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Mendel’s Experimental Results copyright cmassengale

Mendel’s Experimental Results

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Did the observed ratio match the theoretical ratio? The theoretical or

Did the observed ratio match the theoretical ratio?

The theoretical or expected

ratio of plants producing round or wrinkled seeds is 3 round :1 wrinkled.
Mendel’s observed ratio was 2.96:1
The discrepancy is due to statistical error.
The larger the sample the more nearly the results approximate to the theoretical ratio.

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Generation “Gap” Parental P1 Generation = the parental generation in a

Generation “Gap”

Parental P1 Generation = the parental generation in a breeding

experiment.
F1 generation = the first-generation offspring in a breeding experiment. (1st filial generation)
From breeding individuals from the P1 generation.
F2 generation = the second-generation offspring in a breeding experiment. (2nd filial generation)
From breeding individuals from the F1 generation.

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Following the Generations Cross 2 Pure Plants TT x tt Results

Following the Generations

Cross 2 Pure Plants TT x tt

Results in all Hybrids Tt

Cross

2 Hybrids get 3 Tall & 1 Short TT, Tt, tt.

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Monohybrid Crosses copyright cmassengale

Monohybrid Crosses

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Trait: Seed Shape Alleles: R – Round r – Wrinkled Cross:

Trait: Seed Shape
Alleles: R – Round r – Wrinkled
Cross: Round seeds x

Wrinkled seeds
RR x rr

P1 Monohybrid Cross

R

R

r

r

Rr

Rr

Rr

Rr

Genotype: Rr
Phenotype: Round
Genotypic Ratio: All alike
Phenotypic Ratio: All alike

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P1 Monohybrid Cross Review Homozygous dominant x Homozygous recessive Offspring all

P1 Monohybrid Cross Review

Homozygous dominant x Homozygous recessive
Offspring all Heterozygous (hybrids)
Offspring

called F1 generation
Genotypic & Phenotypic ratio is ALL ALIKE

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Trait: Seed Shape Alleles: R – Round r – Wrinkled Cross:

Trait: Seed Shape
Alleles: R – Round r – Wrinkled
Cross: Round seeds x

Round seeds
Rr x Rr

F1 Monohybrid Cross

R

r

r

R

RR

rr

Rr

Rr

Genotype: RR, Rr, rr
Phenotype: Round & wrinkled
Gen. Ratio: 1:2:1
Phen. Ratio: 3:1

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F1 Monohybrid Cross Review Heterozygous x heterozygous Offspring: 25% Homozygous dominant

F1 Monohybrid Cross Review

Heterozygous x heterozygous
Offspring: 25% Homozygous dominant RR 50% Heterozygous Rr 25%

Homozygous Recessive rr
Offspring called F2 generation
Genotypic ratio is 1:2:1
Phenotypic Ratio is 3:1

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What Do the Peas Look Like? copyright cmassengale

What Do the Peas Look Like?

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…And Now the Test Cross Mendel then crossed a pure &

…And Now the Test Cross

Mendel then crossed a pure & a

hybrid from his F2 generation
This is known as an F2 or test cross
There are two possible testcrosses: Homozygous dominant x Hybrid Homozygous recessive x Hybrid

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Trait: Seed Shape Alleles: R – Round r – Wrinkled Cross:

Trait: Seed Shape
Alleles: R – Round r – Wrinkled
Cross: Round seeds x

Round seeds
RR x Rr

F2 Monohybrid Cross (1st)

R

R

r

R

RR

Rr

RR

Rr

Genotype: RR, Rr
Phenotype: Round
Genotypic Ratio: 1:1
Phenotypic Ratio: All alike

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Trait: Seed Shape Alleles: R – Round r – Wrinkled Cross:

Trait: Seed Shape
Alleles: R – Round r – Wrinkled
Cross: Wrinkled seeds x

Round seeds
rr x Rr

F2 Monohybrid Cross (2nd)

r

r

r

R

Rr

rr

Rr

rr

Genotype: Rr, rr
Phenotype: Round & Wrinkled
G. Ratio: 1:1
P.Ratio: 1:1

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F2 Monohybrid Cross Review Homozygous x heterozygous (hybrid) Offspring: 50% Homozygous

F2 Monohybrid Cross Review

Homozygous x heterozygous (hybrid)
Offspring: 50% Homozygous RR or rr 50%

Heterozygous Rr
Phenotypic Ratio is 1:1
Called Test Cross because the offspring have SAME genotype as parents.

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Practice Your Crosses Work the P1, F1, and both F2 Crosses

Practice Your Crosses

Work the P1, F1, and both F2 Crosses for

each of the other Seven Pea Plant Traits.

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Mendel’s Laws copyright cmassengale

Mendel’s Laws

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Results of Monohybrid Crosses Inheritable factors or genes are responsible for

Results of Monohybrid Crosses

Inheritable factors or genes are responsible for all

heritable characteristics.
Phenotype is based on Genotype.
Each trait is based on two genes, one from the mother and the other from the father.
True-breeding individuals are homozygous ( both alleles) are the same.

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Law of Dominance In a cross of parents that are pure

Law of Dominance

In a cross of parents that are pure

for contrasting traits, only one form of the trait will appear in the next generation.
All the offspring will be heterozygous and express only the dominant trait.
RR x rr yields all Rr (round seeds)


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Law of Dominance copyright cmassengale

Law of Dominance

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Law of Segregation During the formation of gametes (eggs or sperm),

Law of Segregation

During the formation of gametes (eggs or sperm), the

two alleles responsible for a trait separate from each other.
Alleles for a trait are then "recombined" at fertilization, producing the genotype for the traits of the offspring.

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Applying the Law of Segregation copyright cmassengale

Applying the Law of Segregation

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Law of Independent Assortment Alleles for different traits are distributed to

Law of Independent Assortment

Alleles for different traits are distributed to

sex cells (& offspring) independently of one another.
This law can be illustrated using dihybrid crosses.

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Dihybrid Cross A breeding experiment that tracks the inheritance of two

Dihybrid Cross

A breeding experiment that tracks the inheritance of two traits.
Mendel’s

“Law of Independent Assortment”
a. Each pair of alleles segregates independently during gamete formation
b. Formula: 2n (n = # of heterozygotes)

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Question: How many gametes will be produced for the following allele

Question: How many gametes will be produced for the following allele arrangements?
Remember:

2n (n = # of heterozygotes)
1. RrYy
2. AaBbCCDd
3. MmNnOoPPQQRrssTtQq

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Answer: 1. RrYy: 2n = 22 = 4 gametes RY Ry

Answer:

1. RrYy: 2n = 22 = 4 gametes
RY Ry rY ry
2.

AaBbCCDd: 2n = 23 = 8 gametes
ABCD ABCd AbCD AbCd
aBCD aBCd abCD abCD
3. MmNnOoPPQQRrssTtQq: 2n = 26 = 64 gametes

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Dihybrid Cross Traits: Seed shape & Seed color Alleles: R round

Dihybrid Cross

Traits: Seed shape & Seed color
Alleles: R round r wrinkled

Y yellow y green

RrYy x RrYy

RY Ry rY ry

RY Ry rY ry

All possible gamete combinations

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Dihybrid Cross copyright cmassengale

Dihybrid Cross

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Dihybrid Cross copyright cmassengale

Dihybrid Cross

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Dihybrid Cross Round/Yellow: 9 Round/green: 3 wrinkled/Yellow: 3 wrinkled/green: 1 9:3:3:1 copyright cmassengale

Dihybrid Cross

Round/Yellow: 9 Round/green: 3 wrinkled/Yellow: 3 wrinkled/green: 1
9:3:3:1

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Test Cross A mating between an individual of unknown genotype and

Test Cross

A mating between an individual of unknown genotype and a

homozygous recessive individual.
Example: bbC__ x bbcc
BB = brown eyes
Bb = brown eyes
bb = blue eyes
CC = curly hair
Cc = curly hair
cc = straight hair

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Test Cross Possible results: copyright cmassengale

Test Cross

Possible results:

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Summary of Mendel’s laws copyright cmassengale

Summary of Mendel’s laws

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Incomplete Dominance and Codominance copyright cmassengale

Incomplete Dominance and Codominance

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Incomplete Dominance F1 hybrids have an appearance somewhat in between the

Incomplete Dominance

F1 hybrids have an appearance somewhat in between the phenotypes

of the two parental varieties.
Example: snapdragons (flower)
red (RR) x white (rr)
RR = red flower
rr = white flower

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Incomplete Dominance r copyright cmassengale

Incomplete Dominance

r

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Incomplete Dominance copyright cmassengale

Incomplete Dominance

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Codominance Two alleles are expressed (multiple alleles) in heterozygous individuals. Example:

Codominance

Two alleles are expressed (multiple alleles) in heterozygous individuals.
Example: blood type
1. type

A = IAIA or IAi
2. type B = IBIB or IBi
3. type AB = IAIB
4. type O = ii

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Codominance Problem Example: homozygous male Type B (IBIB) x heterozygous female Type A (IAi) copyright cmassengale

Codominance Problem

Example: homozygous male Type B (IBIB)
x heterozygous female Type A (IAi)

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Another Codominance Problem Example: male Type O (ii) x female type AB (IAIB) copyright cmassengale

Another Codominance Problem

Example: male Type O (ii) x female type AB

(IAIB)

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Codominance Question: If a boy has a blood type O and

Codominance

Question: If a boy has a blood type O and his sister

has blood type AB, what are the genotypes and phenotypes of their parents?
type O (ii) X type AB (IAIB)
boy girl

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Codominance Answer: Parents: genotypes = IAi and IBi phenotypes = A and B copyright cmassengale

Codominance

Answer:

Parents:
genotypes = IAi and IBi
phenotypes = A and B

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Sex-linked Traits Traits (genes) located on the sex chromosomes. Sex chromosomes

Sex-linked Traits

Traits (genes) located on the sex chromosomes.
Sex chromosomes are X

and Y
XX genotype for females
XY genotype for males
Many sex-linked traits carried on X chromosome.

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Sex-linked Traits Sex Chromosomes Example: Eye color in fruit flies copyright cmassengale

Sex-linked Traits

Sex Chromosomes

Example: Eye color in fruit flies

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Sex-linked Trait Problem Example: Eye color in fruit flies (red-eyed male)

Sex-linked Trait Problem

Example: Eye color in fruit flies
(red-eyed male) x

(white-eyed female) XRY x XrXr
Remember: the Y chromosome in males does not carry traits.
RR = red eyed
Rr = red eyed
rr = white eyed
XY = male
XX = female

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Sex-linked Trait Solution: 50% red eyed female 50% white eyed male copyright cmassengale

Sex-linked Trait Solution:

50% red eyed female
50% white eyed male

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Female Carriers copyright cmassengale

Female Carriers

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Genetic Practice Problems copyright cmassengale

Genetic Practice Problems

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Breed the P1 generation tall (TT) x dwarf (tt) pea plants copyright cmassengale

Breed the P1 generation

tall (TT) x dwarf (tt) pea plants

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Solution: tall (TT) vs. dwarf (tt) pea plants copyright cmassengale

Solution:

tall (TT) vs. dwarf (tt) pea plants

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Breed the F1 generation tall (Tt) vs. tall (Tt) pea plants copyright cmassengale

Breed the F1 generation

tall (Tt) vs. tall (Tt) pea plants

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