Effect of copper-zinc pyrite ore on the processes. Of free radical oxidation in kidney tissue

Сентябрь 4, 2022

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Effect of copper-zinc pyrite ore on the processes. Of free radical oxidation in kidney tissue

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2. Employees of the mining industry have constant contact with the ore, so the problem of intoxication
3. S 15-45% Zn 5% As ≈3% Cu 3,5% Pb 0,2% Cd Cr Fe Mg Other elements
4. Heavy metals content in the body and, accordingly, their influence on different organs and systems in
5. One of the pathogenetic links, when the body is exposed by the ore components, is a
6. The aim of our research was to study the state of free radical oxidation in the
7. Material and methods: The research was carried out on 40 outbreeding male rats, 230±15 g weight.
8. The kidneys were removed and homogenized for 5 minutes at t°- 4°C. The ratio was 1.0
9. The device HL-003 was used for registration processes of free radical oxidation arising from the interaction
10. Results and their discussion
11. Note: the reliability of differences in the values of the experimental groups on the 10th, 20th
12. Record CL homogenate of rats kidneys in the control (1) and experimental groups: for 10 days
13. ROS Oxidative stress
14. Conclusions. The injection of copper-zinc pyrite ore to rats caused an increase in the chemiluminescence of
15. My personal contribution in this scientific research: 1) Care of rats. 2) Decapitation and all surgical
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Employees of the mining industry have constant contact with the ore,

so the problem of intoxication of workers with heavy metals continues to maintain its relevance.

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S
15-45%
Zn
5%
As
≈3%
Cu
3,5%
Pb
0,2%
Cd
Cr
Fe
Mg
Other elements
The ore

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Heavy metals content in the body and, accordingly, their influence on

different organs and systems in persons who have contact with ore. The influence of its components launches the pathophysiological process in bone tissue, liver and other organs.

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One of the pathogenetic links, when the body is exposed by

the ore components, is a change in the state of free radical oxidation (FRO), it is the formation of reactive oxygen species (ROS), which initiate lipid peroxidation (POL). The kidneys are an important organ of elimination, however, the changes occurring in the renal tissue, when it is intoxicated by ore is not enough learned.

Cell

Antioxidant

Free radical

Electron

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The aim of our research was to study the state of

free radical oxidation in the tissue of the kidneys of rats with copper-zinc pyrite intoxication.

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Material and methods: The research was carried out on 40 outbreeding

male rats, 230±15 g weight. They were orally fed with comminuted into powder copper-zinc pyrite ore, which was gotten from Uchalinsky mining plant. Rats were divided into 4 groups depending on the time of exposure of the ore.
We worked in program "Statistics" on the computer, using Mann Withny method.

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The kidneys were removed and homogenized for 5 minutes at t°-

4°C. The ratio was 1.0 g of tissue per 5 ml of phosphate buffer. Further dilution was carried out in phosphate buffer to a ratio of 1 mg/ml, then 20 ml were taken, which placed in the chamber of the luminomer, and luminescence initiated by the addition of 1 ml of a 50 mM solution of ferrous sulfate was registered.

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The device HL-003 was used for registration processes of free radical

oxidation arising from the interaction of free radicals.

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Results and their discussion

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Note: the reliability of differences in the values of the experimental

groups on the 10th, 20th and 30th day of the experiment in relation to the control group with * - p≤0,05 and ** - p≤0,01.

Luminescence values in rat kidney homogenate.

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Record CL homogenate of rats kidneys in the control (1) and

experimental groups: for 10 days (2) and 30 days of research (3).

1.Control group

2.Group 10 days

3.Group 30 days

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ROS
Oxidative stress

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Conclusions.
The injection of copper-zinc pyrite ore to rats caused an increase

in the chemiluminescence of the kidney’s homogenate, starting from the 10th day of the research, remaining at a high level up to 30 days, which testifies to the acceleration of lipid peroxidation processes in the renal tissue.

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