Genome Editing

Июль 31, 2022

Содержание

2. CRISPR Clustered Regularly Interspaced Short Palindromic Repeats Короткие палиндромные кластерные повторы, или CRISPR
12. Two major domains RuvC HNH
15. Variety of CRISPR system
16. CRISPR design tool
17. W.-H. Wu et al., 2016. CRISPR repair reveals causative mutation in a preclinical model of retinitis
18. K. A. Schaefer et al., 2017. Unexpected mutations after CRISPR-Cas9 editing in vivo 117 insertions/delitions 1397
19. TALEN transcription activator-like nuclease
20. From Xanthomonas genus – crops pathogen
21. TALE proteins are composed of a central domain responsible for DNA binding, a nuclear localization signal,
22. DNA-binding domain was demonstrated to consist of monomers, each of them binds one nucleotide in the
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CRISPR Clustered Regularly Interspaced Short Palindromic Repeats Короткие палиндромные кластерные повторы, или

CRISPR

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Two major domains
RuvC
HNH

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Variety of CRISPR system

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CRISPR design tool

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W.-H. Wu et al., 2016. CRISPR repair reveals causative mutation in

a preclinical model of retinitis pigmentosa.

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K. A. Schaefer et al., 2017. Unexpected mutations after CRISPR-Cas9 editing

in vivo

117 insertions/delitions
1397 SNP

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TALEN transcription activator-like nuclease

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From Xanthomonas genus – crops pathogen

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TALE proteins are composed of a central domain responsible for DNA

binding, a nuclear localization signal, and a domain that activates the target gene transcription

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DNA-binding domain was demonstrated to consist of monomers, each of them

binds one nucleotide in the target nucleotide sequence

Monomers are tandem repeats of 34 amino acid residues, two of which are located at positions 12 and 13 and are highly variable (repeat variable diresidue, RVD), and it is they that are responsible for the recognition of a specific nucleotide

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